US 301, I 75 $south
232.8 mi3 hours 47 mins
| Head south on North Lee Street (US 17) | 200 feet | |
| Turn left onto East King Avenue (GA 40) | 1.9 mi | |
| Turn left towards I 95 South: Jacksonville | 0.4 mi | |
| Merge left onto I 95 S | 23.3 mi | |
| Take the ramp towards I 295 West Beltway: Orange Park | 0.6 mi | |
| Continue onto I 295 S | 13.8 mi | |
| Take the ramp towards I-10 West | 0.4 mi | |
| Merge left onto I 10 W | 12.6 mi | |
| Take the ramp towards US 301: Baldwin | 0.2 mi | |
| Keep right at the fork | 600 feet | |
| Merge left onto US 301 | 36.7 mi | |
| Keep left onto Sid Martin Highway (US 301) | 39.1 mi | |
| Turn right onto Northwest 70th Street (FL 326) | 1.7 mi | |
| Continue onto NW 77th Street (FL 326) | 0.9 mi | |
| Continue onto CR 326 | 0.2 mi | |
| Take the ramp on the left | 0 feet | |
| Turn left onto CR 326 | 1,000 feet | |
| Take the ramp | 0.3 mi | |
| Merge left onto I 75 S | 29.1 mi | |
| Keep right towards I 75 South: Tampa | 53.5 mi | |
| Keep right towards I 275 South: Airport | 15.9 mi | |
| Take the ramp towards Downtown East-West | 0.6 mi | |
| Keep left towards Downtown East | 900 feet | |
| Continue onto North Orange Avenue | 0.3 mi | |
| Turn right onto East Cass Street | 0.3 mi | |
| Turn left onto North Ashley Drive | 0.3 mi | |
| Make a U-turn and continue on North Ashley Drive | 100 feet | |
| You have arrived at your destination |
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Kingsland, Georgia - Tampa, Florida