US 14; US 61, US Highway 61
120.3 mi2 hours 26 mins
| Head northwest on Main Street | 200 feet | |
| Turn left onto 3rd Street South (US 53) | 0.3 mi | |
| Continue onto South Avenue (US 53) | 400 feet | |
| Continue onto 3rd South Avenue (US 14) | 0.7 mi | |
| Continue onto South Avenue (US 14) | 1.5 mi | |
| Continue onto Mormon Coulee Road (US 14) | 13.5 mi | |
| Continue onto US 14 | 28.2 mi | |
| Turn right onto Soldiers Grove Road (US 61) | 1.6 mi | |
| Continue onto US Highway 61 (US 61) | 21.4 mi | |
| Turn left onto US 61 | 22.0 mi | |
| Turn left onto State Highway 129 (WI 129) | 2.7 mi | |
| Turn left onto US Highway 61 (US 61) | 16.4 mi | |
| Continue onto West Main Street (US 61) | 1.1 mi | |
| Continue onto Great River Road (US 61) | 900 feet | |
| Take the ramp on the right onto Great River Road (US 61) | 8.1 mi | |
| Continue onto Dubuque-Wisconsin Bridge (US 61 S) | 1.7 mi | |
| Take the ramp towards US 52 North: 9th Street | 0.2 mi | |
| Go straight onto East 11th Street | 0.3 mi | |
| Turn left onto Central Avenue (US 52) | 0.4 mi | |
| Turn left onto East 5th Street | 100 feet | |
| Turn right | 0 feet | |
| You have arrived at your destination |
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La Crosse, Wisconsin - Dubuque, Iowa