I 22 $east; US 78 $east, I 75 $south
651.6 mi10 hours 39 mins
| Head east on Poplar Avenue | 1.1 mi | |
| Turn right onto North Pauline Street | 1.0 mi | |
| Turn left onto East E H Crump Boulevard (US 78) | 800 feet | |
| Continue onto Lamar Avenue (US 78) | 226.6 mi | |
| Keep right towards I 65 South: Birmingham | 0.5 mi | |
| Merge left onto I 65 S | 2.0 mi | |
| Take the ramp towards Finley Boulevard | 1,000 feet | |
| Turn left onto Finley Boulevard (SR 378) | 0.7 mi | |
| Turn right onto Carraway Boulevard (US 31) | 1.5 mi | |
| Continue onto Red Mountain Expressway (US 31 S) | 2.9 mi | |
| Take the ramp onto US 280 | 108.9 mi | |
| Take the ramp on the left onto US 280 | 3.2 mi | |
| Take the ramp towards US 280: Opelika | 0.2 mi | |
| Keep right onto US 280 | 300 feet | |
| Merge left onto Columbus Parkway (US 280) | 21.1 mi | |
| Continue onto US 280 | 6.0 mi | |
| Continue onto Victory Drive (US 27) | 7.4 mi | |
| Continue onto US 27 S | 7.4 mi | |
| Continue onto US 27 | 1.5 mi | |
| Continue onto US 280 E | 18.7 mi | |
| Continue onto Wall Street (GA 520) | 16.1 mi | |
| Continue onto North Parrott Street (GA 520) | 1.0 mi | |
| Continue onto Columbus Highway (GA 520) | 7.9 mi | |
| Continue onto Martin Luther King Jr Drive (GA 45) | 0.5 mi | |
| Continue onto Roundtree Drive (GA 45) | 0.2 mi | |
| Continue onto Vine Street Northwest (GA 45) | 0.3 mi | |
| Continue onto Roundtree Drive Southwest (GA 45) | 400 feet | |
| Continue onto Rountree Drive Southwest (GA 45) | 0.5 mi | |
| Continue onto Forrester Drive Southeast (US 82) | 1.1 mi | |
| Continue onto Albany Highway (US 82) | 5.4 mi | |
| Go straight onto Highway Street (US 82) | 1.0 mi | |
| Continue onto Albany Highway (US 82) | 7.6 mi | |
| Continue onto Warrington Road (US 82) | 1.2 mi | |
| Keep right onto Liberty Expressway (US 82 E) | 8.1 mi | |
| Take exit 3 towards US 82 East: Sylvester | 0.4 mi | |
| Turn left onto Clark Avenue (US 82) | 5.4 mi | |
| Merge left onto Sylvester Highway (US 82) | 14.9 mi | |
| Continue onto US 82 | 17.0 mi | |
| Take the ramp | 0.4 mi | |
| Merge left onto I 75 S | 145.2 mi | |
| Take the ramp towards Gainesville | 0.2 mi | |
| Turn right | 100 feet | |
| Turn right onto West Newberry Road (FL 26) | 2.4 mi | |
| Continue onto West University Avenue (FL 26) | 200 feet | |
| Keep left onto West University Avenue (FL 26) | 3.5 mi | |
| You have arrived at your destination |
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Memphis, Tennessee - Gainesville, Florida